Report on the Bacteriology of Water. 429 



Uroth tube. 



(85.) Unsterilised Typhoid-infected Thames, FlasJc 1 I. (Typhoid 



Absent.) 



The plate exhibited many liquefying colonies as well as a 

 large number of very small colonies ; the latter were placed on 

 potatoes, and yielded light brown growths ; on inoculation into 

 gelatine tubes liquefaction took place, therefore certainly not 

 typhoid. 



(86.) Unsterilised Typhoid-infected Thames, Flask 1 B. (Typhoid 



Absent.) 



The small colonies on the plate which alone exhibited any 

 resemblance to typhoid were examined as in No. 85 above, and 

 yielded exactly similar results, therefore certainly not typhoid. 



(87.) Unsterilised Coli-infected Thames, FlasJc 1 Incubator. (B. coli 



Present.) 



Almost pure cultivation, with numerous typical extension 

 colonies like B. coli ; these yielded characteristic growth on 

 potatoes, gave the indol reaction, and the gas-bubbles in gelatine 

 tube. B. coli, therefore, present. 



(88.) Unsterilised Coli-infected Thames, Flask 1 Refrigerator. (B. 

 coli Present.) 



Exactly similar results with this as with No. 87 above. 

 B. coli, therefore, present. 



From these examinations it appears that on 14.6.1893, 



1. The typhoid bacillus was no longer demonstrable in the Unsterilised 

 Thames water, which had been infected with it thirty-four days pre- 

 viously. It had disappeared both in that portion of the water which had 

 been preserved at a winter, as well as in that kept at a summer, tempera- 

 ture. 



2. The B. coli communis, on the other hand; was easily demonstrable 

 in similar water which had been preserved under precisely the same con- 

 ditions. 



These infected Unsterilised Thames waters were again submitted 

 to examination on 21.6.1893, with the following results : 



2 o 2 



