Report on the Bacteriology of Water. 



431 



Thus, on this day (21.6.1893) as on the occasion of the previous 

 examination (14.6.1893), the typhoid 'bacilli were no longer demonstrable 

 in the unsterilised Thames water into which they had been introduced on 

 11.5.1893. The B. coli communis, on the other hand, was again easily 

 discoverable under the same circumstances, i.e., forty days after its intro- 

 duction into the unsterilised Thames water. 



The typhoid-infected unsterilised Thames water was again simi- 

 larly examined on 26.6.1893, with the following results : 



Examination by Phenol Broth-culture on 26.6.1893. 



The plate cultivations made from the above phenol broth tubes 

 yielded the following results : 



Broth tube. 



(96.) Typhoid-infected Unsterilised Thames, Flask \ Incubator. 

 (Typhoid Absent.) 



The plates contained some small colonies presenting some 

 resemblance to typhoid ; on being transferred to potatoes they 

 yielded light brown growths unlike typhoid, and on being 

 inoculated into gelatine green fluorescence without liquefaction 

 was obtained. These colonies were, therefore, not those of 

 typhoid. 



(97.) Typhoid-infected Unsterilised Thames, Flask 1 Refrigerator. 

 (Typhoid Absent.) 



The plates exhibited two types of colony, firstly, liquefying 

 ones which could not be typhoid, and, secondly, small depth 

 colonies, which on transference to potatoes gave thick greyish- 

 brown growths wrinkled in parts, and on inoculation into 

 gelatine tubes caused subsequent liquefaction. These were, 

 therefore, not typhoid. 



