432 



Profs. Percy Frankland and Marshall Ward. 



This examination, made on 26.6.1893, confirmed, therefore, the two 

 previous examinations of 21.6.1893 and 14.6.1893, which showed that 

 the typhoid bacillus, introduced on 11.5.1893, was no longer discoverable 

 in the unsterilised Thames water. 



The final examination of these waters was made on 5.7.1893, 

 thus 



Examination by Phenol Broth-culture on 5.7.1893. 



Thus, whilst the broth tubes containing only 3 drops of phenol 

 still became turbid when inoculated with both uninfected and in- 

 fected waters, the broth tubes to which 5 drops of the phenol solution 

 were added remained clear after inoculation with both waters. 



The plate cultivations made from the above broth-tubes, which 

 became turbid, yielded the following results : 



Broth tube. 

 (106.) Unsterilised Uninfected Thames, Flask I Incubator. 



The plates exhibited a large number of highly fluorescent 

 expansion colonies without liquefaction ; some of the least 

 fluorescent of these colonies, and bearing therefore a faint 

 resemblance to typhoid, were transferred to potatoes, on which 

 they gave rise to light brown, sharply demarcated growths, 

 quite unlike typhoid. No gas bubbles were produced on in- 

 oculation into melted gelatine tubes. 



