Report on the Bacteriology of Water. 433 



Broth tube. 

 (107.) Unsterilised Uninfected Thames Flask 1 Refrigerator. 



The plates exhibited an apparently pure cultivation of a 

 liquefying organism, the colonies having a strong resemblance 

 to those of B. liquidus (see 2nd Report, p. 186). Some of the 

 colonies which had not yet caused liquefaction were trans- 

 ferred to potatoes, on which they gave rise to thick, whitish, 

 waxy growths, quite unlike those of typhoid. 



(108.) Typhoid-infected Unsterilised Thames, Flask 1 Inctibator. 

 (Typhoid Absent.) 



The plates contained fluorescent expansion, liquefying and 

 small dot colonies. All three types of colony were transferred 

 to potatoes, on which the small dot and fluorescent expansion 

 colonies gave rise to light brown sharply marked growths, and 

 the liquefying colonies to shining slimy colourless growths ; 

 none of these were, therefore, similar to those of typhoid. 



(109). Typhoid-infected Unsterilised Thames, Flask 1 Refrigerator, 

 (Typhoid Absent.} 



The plates appeared to be pure cultivations ; the depth 

 colonies were small dots, often oval in shape, and the surface 

 colonies resembled very small " milk-drops ; " even on the plate 

 in which the colonies were few and far between there were 

 none of the typical expansion colonies resembling typhoid. 

 On transference to potatoes, shining slimy growths, having 

 the appearance of drops of water, were obtained. 



This final examination of these Unsterilised typhoid-infected 

 Thames waters therefore again confirmed the previous results, which 

 may be thus summarised: 



(1.) The Unsterilised uninfected Thames water, collected at 

 Hampton on 5.5.1893, contained throughout the 

 entire course of the series of experiments no bacteria 

 resembling either the typhoid bacillus or the B. coli 

 commtmis. 



(2.) The Unsterilised Thames water infected with typhoid 

 on 11.5.1893, was still found to contain living typhoid 

 bacilli on 5.6.1893, or twenty-five days after infection, 

 whilst on 14.6.1893, thirty-four days after infection, 

 they were no longer demonstrable. 



(3.) These remarks apply equally to the waters preserved at 

 a winter and a summer temperature respectively, 



