Report on the Bacteriology of Water. 

 Examination by Phenol Broth-culture, 29.5.1893. 



439 



Thus, the only saline water which gave a positive reaction with the 

 phenol broth test was the one in which an addition of only O'l per 

 cent, of salt had been made, and, even in this case, it was only the 

 flask which had been kept at the winter temperature of 6 8 C. in 

 the refrigerator, whilst the corresponding flask preserved at the 

 summer temperature of 19 C. gave a negative result. Plate cultiva- 

 tions were made of the turbid broth-tube No. 11, and these yielded 

 the characteristic typhoid colonies, which were further confirmed by 

 potato growth, and negative results with the indol and gas-bubble tests. 

 On referring to the results of phenol broth-culture (pp. 424 434) 

 obtained with the corresponding waters to which no salt had been 

 added, it will be seen that they present a great contrast to these, as 

 both the incubator and refrigerator flasks of the typhoid-infected 

 Unsterilised Thames water gave a positive reaction, and were found 

 to contain living typhoid bacilli. 



From these examinations ii appears then that on 29.5.1893, or eighteen 



