Report on the Bacteriology of Water. 447 



Broth tube. 



(98.) Typhoid-infected Unsterilised Thames + Ol per cent. NaCl, 

 Flask 1 Incubator. (Typhoid Absent.) 



The plates exhibited liquefying and small colonies respec- 

 tively. From the small colonies, potatoes were inoculated, a 

 brown and slimy growth being obtained, on inoculation from 

 which into gelatine tubes, a blue, fluorescent, non- liquefy ing 

 growth resulted. Thus nothing like typhoid was present. 



(101.) Typhoid-infected Unsterilised Thames + O'l per cent. N"aCl, 

 FlasJc 1 Refrigerator. (Typhoid Absent.) 



The potato-growths obtained from the colonies bore some 

 resemblance to typhoid, although the surface was rather too 

 shining. On inoculating into gelatine tubes, it was found that 

 very slow liquefaction took place. This was, therefore, the same 

 organism which had been several times before met with in this 

 flask. Under the microscope the bacilli also present some re- 

 semblance to typhoid, but they have squarer ends. 



(99.) Typhoid-infected Unsterilised Thames + 1 per cent. NaCl, 

 Flask 1 Incubator. (Typhoid Absent.) 



On the plates there were small colonies forming surface ex- 

 pansions with green fluorescence ; these gave rise, on potatoes, 

 to thick, greyish-brown growths quite unlike typhoid. 



(102.) Typhoid-infected Unsterilised Thames + 1 per cent. NaCl, 

 Flask 1 Refrigerator. (Typhoid Absent.) 



The plates contained both liquefying and small colonies, the 

 latter, on transferring to potatoes, gave strong, conspicuous, 

 and flesh-coloured growths quite unlike typhoid. 



(100.) Typhoid-infected Unsterilised Thames + 3 per cent. NaCl, 



Flask 1 Incubator. (Typhoid Absent.) 



The plates contained small colonies forming surface expan- 

 sions, which gave light brown growths on potatoes quite unlike 

 typhoid. 



(103.) Typhoid-infected Unsterilised Thames + 3 per cent. Nad, 

 Flask 1 Refrigerator. (Typhoid Absent.) 



The plates contained liquefying and small colonies respec- 

 tively, the potato-growths from the latter were strong, thick, 

 waxy, and greyish- white, quite unlike typhoid. 



Thus, on this occasion (26.6.1893) again, the presence of typhoid 

 bacilli could not be demonstrated in any of these saline waters. 



The final examination of these saline waters was made on 5.7.1893, 

 with the following results : 



