Report on the Bacteriology of Water. 449 



colonies, on being transferred to potatoes, again yielded incon- 

 spicuous colourless growths very similar to those of typhoid. 

 Negative results were also obtained with the milk, indol, and 

 gas-bubble reactions. It was found, however, that the 

 gelatine-tubes inoculated from these typhoid-like colonies 

 underwent very slow liquefaction. This was, therefore, ob- 

 viously the same organism which had been repeatedly 

 obtained before from this flask, and the superficia? resem- 

 blance to typhoid of which has been already referred to (see 

 pp. 445, 447). 



Broth tube. 



(114.) Typhoid-infected Unsterilised Thames + 1 per cent. NaCl, 

 Flask 1 Incubator. (Typhoid Absent.) 



The plates contained fluorescent non-liquefying colonies. 

 The less obviously fluorescent colonies on transference to 

 potatoes yielded growths which were not sufficiently different 

 from typhoid to decide, whilst the milk, indol, and gas-bubble 

 tests were also negative. On inoculation into gelatine tubes, 

 however, the latter became fluorescent. 



(117.) Typhoid-infected Unsterilised Thames + 1 per cent. 

 Flask 1 Refrigerator. (Typhoid Absent.) 



The plates exhibited a pure cultivation of bacillus, giving 

 rise to small, cup-shaped, liquid colonies, probably 13. liquidus 

 (Percy Frankland), which is very frequently found to survive 

 in the 3-drop phenol broth-cultures. 



(115.) Typhoid-infected Unsterilised Thames + 3 per cent. NaCl, 

 Flask 1 Incubator. (Typhoid Absent.) 



The plates exhibited small, milk-drop colonies, with slight 

 tendency to expand. Microscopic examination showed them 

 to be due to bacilli thinner than the typhoid bacillus, and on 

 potatoes they yielded light yellow, shining growths, unlike 

 those of typhoid. 



(118.) Typhoid-infected Unsterilised Thames + 3 per cent. NaCl, 

 Flask 1 Refrigerator. (Typhoid Absent.) 



The plates contained a number of liquefying colonies, 

 apparently B. liquidus (Percy Frankland), also some small 

 colonies, some of which gave rise to very small surface ex- 

 pansions and very small milk-drops. The potatoes inoculated 

 from the milk-drop colonies yielded flesh-coloured growths, 

 whilst those from the expansion colonies were not decisive. 



