456 Profs. Percy Frankland and Marshall Ward. 



Examination by Phenol Broth-culture on 5.6.1893. 



These examinations by phenol broth-culture show, therefore, that, 

 on 5.6.1893, the typhoid-infected steam-sterilised Thames water 

 reacted already in twenty-four hours with the test, irrespectively of 

 whether 3 drops or 5 drops of phenol solution were added to the 

 10 c.c. of broth; whilst, by referring back to p. 427, it will be seen 

 that the typhoid-infected unsterilised Thames water only reacted in 

 twenty- four hours, even with the 3 drops of phenol solution, in the case 

 of the water which had been kept at the winter temperature, whilst 

 the summer temperature water only reacted after forty-eight hours. 

 From these comparative tests it can be inferred, therefore, that, on 

 the date in question, the typhoid bacilli were in a less vigorous con- 

 dition in the unsterilised than in the sterilised water. 



Of the plate cultivations made from the turbid broth tubes Nos. 

 61, 57, and 59, those from No. 61 yielded typical typhoid colonies 

 which were confirmed by growth on potatoes and by negative results 

 with the indol and gas-bubble tests ; the plates from Nos. 57 and 59, 



