474 



Profs. Percy Frankland and Marshall Ward. 



Examination of Unsterilised Loch Katrine Waters by Phenol Broth- 

 culture, 15.7.1893. 



Of the plate cultivations made from the above turbid broth-tubes, 

 it will be sufficient to say : 



1. That from the uninfected water only liquefying colonies were 

 obtained, probably B. liquidus (Percy Frankland) ; as already men- 

 tioned, this organism is very frequently obtained in phenol broth- 

 cultivations in which only 3 drops of phenol solution has been added. 



2. The plates from the phenol broth-tube which had only been 

 rendered turbid in seventy-two hours by the incubator flask of the 

 typhoid-infected water, yielded also only liquefying colonies, and 

 nothing like typhoid colonies was discoverable on the plates. The 

 corresponding broth-tube from the refrigerator flask, on the other 

 hand, which had become turbid in twenty-four hours, yielded numer- 

 ous small and expansion colonies which were undoubtedly due to 

 typhoid. 



