/>3S Profs. Percy Frankland and Marshall Ward. 



Steam-sterilised Peaty Water, infected 30.10.1893 with continued. 



Typhoid bacillus. Plate cultivations made _B. coli communis. 



6.11.1893 



, ^ 



1044 colonies from 14,031 colonies from 



1 c.c. (plates 1 c.c. (plates 



incubated 7 days). incubated 7 days). 



15.11.1893 



432 colonies from 17,580 colonies from 



1 c.c. (plates 1 c.c. (plates 



incubated 5 days). incubated 5 davs). 



23.11.1893 



220 colonies from 14,457 colonies from 



1 c.c. (plates 1 c.c. (plates 



incubated 6 days). incubated 4 days). 



Peaty Water Sterilised by Filtration, infected 30.10.1893, with 



Typhoid bacillus. Plate cultivations made S. coli communis. 



3010.1893 



, . _^ 



1169 colonies from 21,603 colonies from 



1 c.c. (plates 1 c.c. (plates 



incubated 6 days). incubated 4 6 days). 



6.11.1893 



r- * : x 



No colonies on plates No colonies on plates 



(plates incubated (plates incubated 



9 days). 9 days). 



Thus even in this water, heavily charged as it was with vegetable 

 organic matter, the typhoid bacilli failed to undergo any multiplication, 

 but, on the contrary, as usual, suffered a continuous numerical decline. 

 The B. coli communis, on the other hand, remained practically unaltered 

 in numbers during the period of upwards of three weeks over which these 

 observations extended, the slight numerical increase being too insignificant 

 to be regarded as evidence of true multiplication. 



In the peaty water which had been- sterilised by filtration through 

 porous porcelain there was again the same phenomenon, so frequently 

 referred to before in this Report, of the extraordinarily rapid disappear- 

 ance of the introduced tijphoid and coli bacilli. 



On the possible Adaptation of the Typhoid Bacillus to active life in 

 Potable Water. 



In none of the experiments recorded above was any multiplication 

 of the typhoid bacillus observed, although these experiments have 

 been made with waters varying from the deep well water of the Kent 

 Company, which is almost wholly destitute of organic matter, to the 

 peaty water referred to on p. 537, which contains about the maximum 

 amount of organic matter met with in water used for drinking 

 .purposes. 



