Report on the Bacteriology of Water. 



551 



The tubes containing this infected water were kept in a dark cup- 

 board at 12 C., and submitted to plate cultivation at intervals, 

 thus : 



These tubes were again examined on two subsequent occasions and 

 again yielded sterile plates. 



Thus these anthrax bacilli, taken directly from the dead mouse and 

 introduced into sterile Dundee water in such large numbers as 9000 per 

 1 c.c. of water, all died within five days, the water being kept at 12 C. 



Second Series of Experiments. 



In this series of experiments the anthrax bacilli from the dead 

 animal were introduced both into steain-sterilised Thames and steam- 

 sterilised Dundee water respectively, and these waters were preserved 

 at different temperatures, in order to ascertain the influence of this 

 factor on the result. 



The spleen of a white mouse, which had died of anthrax twenty- 

 eight hours after inoculation, was broken up with sterilised tap water 

 in the same way as already described above, and with this water 

 attenuation larger volumes of sterile Thames and Dundee waters 

 respectively were infected, and these infected waters were then dis- 

 tributed amongst a number of sterile tubes plugged with cotton wool. 

 On the day of infection some of these tubes were submitted to plate 

 cultivation, in order to ascertain the number of anthrax bacilli 

 introduced, thus : 



f Tube No. 1 j J c-c- 

 Dundee waters 



I 



Thames water < 

 i. 



'{ft 



water yielded 4840 anthrax colonies per 1 c.c. 

 4860 

 6897 



7722 



6136 



5875 

 6480 

 6552 



