CHAPTER IX 

 PANCREATIC DIGESTION. BILE 



Pancreatic Juice 



A solution of pancreatic ferments may be prepared by ex- 

 tracting a pig's pancreas with glycerine or with water contain- 

 ing chloroform. For laboratory purposes it is, however, more 

 convenient to use a solution of commercial pancreatic powder. 



Composition of Pancreatic Juice. 



Natural pancreatic juice contains small amounts of proteins 

 and other organic substances. 



Inorganic salts, chiefly sodium carbonate. 



Ferments. The active pancreatic juice contains three im- 

 portant enzymes : trypsin, amylase, and lipase. 



Digestive Action. 



On proteins. 



1. Prepare four test tubes as follows: 



a. 5 c.c. neutral pancreatic solution. 



d. 5 c.c. neutral pancreatic solution + 2 drops saturated so- 

 dium carbonate solution. This gives a concentration of about 

 0.2% sodium carbonate. 



c. 5 c.c. neutral pancreatic solution + 3 to 4 drops of 10% 

 HC1. This gives a concentration of about 0.2% acid. 



d. 5 c.c. neutral pancreatic solution -f- 2 drops saturated so- 

 dium carbonate solution, boil thoroughly 3 or 4 minutes and 

 cool. 



To each tube add a Mett tube as described under "Gastric 

 Digestion" and incubate until the next period. 

 Examine the tubes and tabulate results. 



Trypsin acts best in a slightly alkaline solution. It also will 



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