Report on the Bacteriology of Water. 



That is to say, this filament doubled its length in fifty-six minutes 

 at 2423-25 C. 



Further measurements were made during the evening, and showed 

 that the rapid growth continued, for at 6.40 P.M. the temperature 

 being 23 C. this filament was approximately 450 fi long, and at 

 7.;W * = 22-5 considerably over 1000 /i. 



At the same time (7.30 P.M.) and at exactly the same temperature 

 the culture over water showed one or two filaments, very feeble and 

 sharply segmented, about 100 ft, long. 



At 10 P.M., temp. 22*5 in both cells, the long coils of the bichro- 

 mate-culture were several thousand ft, long, whereas the feebly growing 

 one or two filaments of the water-culture measured from 150 to 250 /t 

 at most, and were much broken up and starved looking. 



Even on July 2, when examined at 8.30 A.M., the water-culture 

 had no filament so long as 500 /*, though one or two were over 400 ;t 

 long, but by this time the filaments of the other culture measured 

 many thousand /*. 



By 9 A.M. on July 3 the bichromate-cnlture was fully grown, and 

 had developed magnificent spores in every filament; the water-culture 

 showed no trace of spores, though the shortly segmented filaments 

 were very granular. 



These facts are of especial interest and importance as disposing 

 effectually of any question as to so weak a food material being 

 unsuitable for the growth, &c., of this bacillus. It is quite clear 

 that very little is needed for normal development. 



On July 4, after another day's exposure but July 3 was dull, hot, 

 and cloudy all day the culture over water was still devoid of spores. 

 After the whole of the 4th, in dark incubator at 22 C., still no signs 

 of spores. 



On July 5 one or two spores were detected at isolated spots 

 here and there in the filaments, but very few. 



