Report on the Bacteriology of Water, 



393 



There was no reason for concluding that the main mass of the 

 filament grew any slower, but it may have been so. 



It scarcely seemed worth while to pursue this, so another filament 

 was taken in the same culture. 



The very slow growth, although at and near 25 C., was confirmed 

 by examining another culture, started at the same time at 22 C., and 

 put iuto Sachs' box at 6.30 P.M. 



Here the curve was actually flattening more and more as time 

 went on. 



Another filament was then taken. 



The growth was so slow that I abandoned the measurements. I 

 cannot explain it, unless the following suggestion is to the point. 

 The cotton-wool plugging the arms had been slightly scorched during 

 sterilisation : my practice is not to heat up to browning point, but in 

 this case that had occurred. Can it be that traces of pyrid ine-like 

 or other bodies get into such a cell, and cause slight antiseptic 

 inhibition ? Ifc would probably be worth somebody's while to make 

 a series of experiments with such cultures to this end, with various 

 volatile bodies added in known doses in the arms of the pells. 



Experiments on the Effects of Temperature only. 



Various results in the foregoing account of my experiments sug- 

 gested that it would be well to obtain more accurate information as 

 to the effect of temperature alone on the growth curves. Of cours > 

 a good deal of work has been done on the influence of temperature 



VOL. LVIII. 2 E 



