Report on the Bacteriology of Water. 



39<> 



On analysing the results, the filament doubled its length, as 

 follows : 



1. 18 36 p in 32 minutes at 24'5 C. 



2. 36 72,, 47 24-75 25 '5 C. 



3. 72144,, 43 25 "5 25 C. 



Reckoned back : 



184 -5 92 -25 fj. = 4.21 P.M.-^5.10 P.M. = 49 minutes at 25 '52525 '25 C. 

 92 -25 46 -0 = 3.35 4.21 = 41 24 '7525 '525 C. 

 46-023'Q ,, = 2.53 3.25 =32 24 '5 25 C. 



Normal doubling periods : 



35 70 ft. = 3.15 P.M. 4.3 P.M. = 48 minutes at 24 '5 25 '5 C. 

 70 140,, = 4.3 ,,4.50 -47 25 '525 C. 



The following are the results of the second series of measurements ; 

 the slight variations in the temperature (not to be overlooked) were 

 due to the necessity of rearranging the culture, and therefore opening 

 the side windows. 



Calculated back : 

 252126 /* = 6 P.M. 7.10 P.M. = 70 minutes at 25 -2524 '7525 -225 C. 



The doubling, from 81 /i to 162 /*, took 59 minutes at temperatures 

 varying between 24'75 and 25-25 C., and, by the shape of the curve, 

 the rate would probably not be far different for the next doubling 

 period. 



This specimen, which had grown vigorously during the night of 

 the llth, was kept at the same temperature, 24' 5 to 25*5, all through 

 the 12th, in the dark. It was necessary to open the chamber at 

 9.30 A.M. on the 12th, and the temperature fell to 22*5 for a few 

 minutes, but it had recovered before 10 A.M. 



