400 Profs. Percy Frankland and Marshall Ward. 



At 9 P.M. it had completed the formation of magnificent series of 

 spores, i.e., in less than 36 hours from sowing, the sporification was 

 complete. 



On July 15 I repeated the observations in the Sachs' box, the sowing 

 being made weak gelatine and put in at 12 noon. 



The temperatures of the box ran as follows, before germination : 



Temp. 



The measurements began at 7.45, and continued till 10.10 P.M., 

 with the following results : 



Doubled its length as follows : 



1. 36 72 n in 50 minutes at 2827-25 C. 



2. 72144 58 27 -2525 '5 C. 



And at its present rate would have again doubled : 

 3. 144288 n in 54 minutes at 25 '5 C. 



Normal doubling periods : 



55110 n = 8.15 P.M. 9.10 P.M. = 55 minutes at 27 -526 C. 

 110220 = 9.10 10.6 = 56 26 '025 '5 C. 



At 9 P.M. on the 16th, the culture having been at 25 to 27-5 

 during that day (and up to 30'5 between 6.20 and 8.30 P.M.), many 

 of the filaments had already formed their spores, i.e., spore-formation 

 had definitely set in in 33 hours. At 8.30 next morning there were 

 fully formed spores in all filaments, large, and very brilliant. 



It is clear that at temperatures near 25-r 27 C. spores may be 

 developed in less than 48 hours. 



This experiment was repeated on the 16th July. The spores, 

 exactly as before, were started at 9.45 C., the temperatures running 

 as follows : 



