Report on the Bacteriology of Water. 



407 



16-9 17 C. 

 17 18 C. 

 18 C. 



78 minutes at 1818 -25 C. 

 80 17 -418 C. 

 82 1717 -4 C. 

 74 17 16-9 17 C. 



Curve 87, p. 402. 

 The doubling periods are here as follows : 



1. 11-2522 -5 n = 79 minutes at 17 '116 '9 C. 



2. 22-5 45 = 86 



3. 45 90 = 79 



4. 90 180 = 78 



.Calculated back : 



270135 ft = 1.42 P.M. 3.0 P.M. 



13567 '5 = 12.22 1.42 

 67 -533 -75 ,, = 11.0 A.M. 12.22 P.M. 

 33-7516-5,,= 9.46 11.0A.M. 



formal doubling periods : 



13 -5 27 p = 9.20 A.M. 10.30 A.M. = 70 minutes at 1716 '9 C. 

 27 54 = 10.30 11.58 = 88 16 -917 C. 

 54108 = 11.58 1.19 P.M. = 79 1718 C. 

 108216,,- 1.19PM. 2.37 =78 18 18 '2 C. 



In the same culture I selected another filament at 4.45 P.M., July 24, 

 and measured from 4.46 P.M. to 6.24 P.M. 



The doubling periods were here as follows : 



85 -5171 -0 n = 77 minutes at 18 '7519 C. 



Calculated back : 



220-5110-25 n = 5.17 P.M. 6.24 P.M. = 67 minutes at 18-7519-5 C. 



Spores were beginning to form in some filaments at 8.0 P.M. on 

 July 26, but, even on 28th, few, sparse, and small spores, much poorer 

 than those of 25th July. 



On July 25 spores in broth were sown at 9.30 A.M., and at once put 

 into Sachs' box, dark, at 19 C. The temperature rose slowly to 19.5 

 at 10.15, and to 20'25 at 2 P.M. ; thence it remained at 20'25 20'75, 

 a rodlet being selected at 3.35 P.M. and kept under observation till 

 6.20 P.M. 



