408 Profs. Percy Frankland and Marshall Ward. 



Doubling periods : 



1. 20 -25 40 '5 n in 49 minutes at 20 '7520 '25 C. 



2. 40-5 81 56 20 -25 20 -6 C. 



3. 81 162 63 20'6 20-5 C. 



Calculated back : 



157 78 -5 p. = 5.18 P.M. 6.20 P.M. = 62 minutes at 20-620 '5 C. 

 78-5 39 '5,, = 4.22 5.18 =56 20 -25 20 '6 C. 



Normal doubling periods : 



33 -75 57-5 p. = 4.0 P.M. 5.6 P.M. = 66 minutes at 20. 520 '2520-6 C. 

 67 -5 135 -0 = 5.6 6.6 = 60 20 -620 -5 C. 



This 25th July culture had formed splendid spores by 10.45 P.M. on 

 the 27th, whence the whole life cycle took about 60 hours at 

 20 23 C., having been in the dark under a bell-jar since 26th, 



9A.M. 



On August 4 a spore culture in broth was started at 7 A.M. at 21 C., 

 rising very slowly, the variations were : 



Temp. 



Germination began about 9.15, as I saw a spore swollen and 

 elongating then ; at 9.45 several were seen from 4 /u, to 6 ft long, and 

 at 10.30 one was selected for measurement 10 p long, as follows : 



