410 



Profs. Percy Frankland and Marshall Ward. 



Spores had begun to round off at 7 A.M. on August 7, i.e., life cycle 

 in less than 24 hours. 



On August 3 spores were sown in a broth-drop at 11.30 A.M., and 

 put to germinate, in the incubator at 23 C. ; they did this rapidly 

 and normally about 3.30, and at 3.50 the culture was put into the 

 Sachs' box. During the accommodation period the temperature fell 

 from 28'6 at 3.50 to 27'25 at 4.40, when a germinating rodlet was 

 selected for measurement, as follows : 



Normal doubling period : 



816 IJL = 5 P.M. 5.44 P.M. = 44 minutes at 27'0 26 -5 C. 

 but as this was the germinating phase, take 



1632 fi = 5.44 P.M. 6.18 PiM. = 34 minutes at 26 -526 -4 C. 



Doubling periods : 



1. 612 fji = 4.40 P.M. 5.29 P.M. = 49 minutes at 27 -2526 '7 C. 



2. 1224 = 5.29 6.3 = 34 26 '7 26 '5 C. 



3. 2448,, = 6.3 6.41 = 38 26 '5 26 '426 5 C. 



4. 4896 = 6.41 7.16 = 35 26 '5 26 -6 C. 



Calculated back : 



103 -5 51 -75 /t = 6.42 P.M. 7.20 P.M. = 38 minutes at 26 '526 '626 '4 C. 

 51 -7526 -0 = 6.6 6.42 = 36 26 -526 '426 '5 C. 

 26-013-0 ,, = 5.33 6.6 =33 ,, 26 '6 26-4 C. 



Another filament, selected at 8.40, gave the following : 



