

Report on the Bacteriology of Water. 411 



Doubling period : 



100-75201 -5 M - 8.43 P.M. 9.26 P.M. - 43 minutes at 28 -2527 C. 

 Or, reckoned back : 



252126 p = 8.58 P.M. 9.45 P.M. = 47 minutes at 28-2527 C. 



On August 2 a broth-culture of spores was started at 21-25 in dark 

 at 9.40 A.M. Germination was very rapid at 21-25 21'5, the tem- 

 perature being nearly constant, and at 1.45 there were rods from 

 45 /t to 70 fi and longer. 



I then rapidly raised the temperature from 21'6 at 1.45 as fol- 

 lows-: 



Temp. 



and selected a rod, in two segments at an angle of 75 or so, for 

 measurement. 



The variations of temperature between 2.10 and 2.40 should be 

 noticed. They were 



Temp. 

 Time. C. 



2.10 P.M. = 29-7 



2.12 = 30-0 1 ,,, , 



o i Q oo.n ( * al1 due to P enin g tQ e box 



j.io ,, = &\) \j j 



2.14 = 29-3 



2.15 = 29-5 



2.16 = 29-7 

 2.18 = 29-9 

 2.20 = 30-0 

 2.25 = 29-9 

 2.30 = 29-9 

 2.40 = 30-0 



From thence onwards the temperature changed very slowly : 

 equilibrium was attained. 



Segment A. 



This filament was followed from 2.10 P.M. to 3.10 P.M. and had then 



2 F 2 



