412 Profs. Percy Frankland and Marshall Ward. 



to be abandoned, owing to the pronounced curvatures often setting 

 in at these higher temperatures. 



Doubling period : 



1836 p. = 2.10 P.M. 2.53 P.M. = 43 minutes at 29 '7 30 29 9 30 C. 

 Calculated back : 



51 -7525-75 n = 2.32 P.M. 3.10 P.M. = 38 minutes at 29-93029-9 C. 

 Normal doubling period : 



24 -7549 -5 ju = 2.30 P.M. 3.9 P.M. = 39 minutes at 29 '93029 '9 C. 



Segment B. 



"Was followed from 2.10 P.M. to 3.10 P.M., and then abandoned. 

 Doubling period : 



1836 n = 2.10 P.M. 3.0 P.M. = 50 minutes at 29 '73029 '930 C. 



Calculated back : 



4522 -5 fj. = 2.30 P.M. 3.10 P.M. = 40 minutes at 29 '93029 '9 C. 



Normal doubling period : 



22 '545 p. = 2.30 P.M. 3.10 P.M. = 40 minutes at 29 '9 30 29 '9 C. 



Total. 

 Doubling period : 



3672 M = 2.10 P.M. 2.57 P.M. = 47 minutes at 29 '7 30 29 '9 30 C. 

 Or, calculated back : 



9648 /* = 2.31 P.M. 3.10 P.M. = 39 minutes at 29 '93029 '9 C. 



Another segment in the same culture was chosen at 3.24 P.M. and 

 followed till 4.10 P.M. 



Doubling period : 

 118236 n = 3.24 P.M. 4.3 P.M. = 39 minutes at 29 29 '75 28 '75 C. 



Or, calculating back : 



260130 \L = 3.28 P.M. 4.10 P.M. = 42 minutes at 2929 75 28 '75 C. 



On August 7 a broth-drop of spores was put into the incubator at 

 23 C. at 7.30 A.M., and left to germinate. At 10.40 there were rods 

 36 ft to 45 fi long, and the culture was put into Sachs' box at 31 C. ; 

 during accommodation the temperatures ran 



Temp. 

 Time. C. 



10.49 A.M. = 29-5 

 11.0 = 31-0 

 11.15 = 31-0 



