420 Profs. Percy Frankland and Marshall Ward. 



Doubling periods calculated back : 



276-75138-5 fi = 3.50 P.M. 3.13 P.M. = 37 minutes at 26-2525-75 C. 

 138 -5 69 -25 = 3.13 2.40 =33 25 '75 26 -25 C. 



Normal doubling period : 



110-25221 n = 3 P.M. 3.37 P.M. = 37 minutes at 2626-2526 C. 



Evidently the temperatures were near the optimum. 

 On July 27 spores sown in broth + 1 per cent, gelatine were put 

 in at 7.45, the temperatures running as follows : 



Temp. 

 Time. C. 



7.45A.M. = 19-5 



8.0 = 20-25 



9.0 = 23-1 



9.45 = 24-5 



10.10 = 24-8 



10.30 = 25-4 



11.7 = 26'0 "1 germination 



11.40 = 26-75 J beginning 



Fell to 26 on opening 



12.30 P.M. = 27-0 



12.45 = 27-0 



1.0 = 27-2 



1.30 = 27-5 



2.0 = 27-5 



The measurements were now begun on a stout filament divided 

 into two segments, which were both very straight, and formed an angle 

 of about 150 with each other. It was easy to measure both segments, 

 practically simultaneously, and so get not only the growth curve of 

 each segment but also (as the sum of the two) that of the whole 

 filament, as follows : 



Segment 1. 

 The doubling period here : 



78 -75157 -5 M in 39 minutes at 27 '527 '7 C. 

 Or, calculated back : 



234127 n = 2.25 P.M. 3.0 P.M. = 35 minutes at 27 '627 '75 C. 



Normal doubling period: 



78-75157 -5 M = 2.0 P.M. 2.39 P.M. = 35 minutes at 27 '527 '9 C. 



