Report on the Bacteriology of Water. 



421 



Segment 2. 

 The doubling period here : 



72144 p. in 38 minutes at 27 '527 '7 C. 



Or, calculated back : 



225112-5 M = 2.24 P.M. 3.0 P.M. = 36 minutes at 27 '6 27 '8 27 *75 C. 



Normal doubling period : 



72144 n - 2.0 P.M. 2.38 P.M. = 38 minutes at 27 '527 '7 C. 



Total. 



The following measurements were made on another filament in this 

 culture : 



In this case the measurements were made with Zeiss C, each scale 

 division = 7'8/t, and I regard the numbers as not quite so accurate as 

 preceding ones, partly owing to the amplification and partly to the 

 extremely rapid growth. 



The doubling period : 



149298 /x in 35 minutes at 2727 '5 C. 



Calculated back : 



343172 -5 M = 40 minutes at 27 '527 "7 C. 



This culture remained in the Sachs' box through all the next day 

 (July 28) at 26 33, and had not developed spores on the 29th at 

 10 A.M. It was returned to the box. On the 30th, spores were formed. 



On August 3 spores sown in broth + 1 per cent, gelatine were put 

 at 5.15 A.M. into the incubator at 23 C. to germinate. At 10.20 the 

 Sachs' box was ready to receive them. Germination had been rapid 

 and normal, and rods from 40 to 70 /* were present. 



The culture was fixed at 10.25 at 24'5 C. 



The temperatures during accommodation varied as follows : 



