422 Profs. Percy Frankland and Marshall Ward. 



Temp. 

 iTime. C. 



10.25 A.M. = 24-5 



10.27 = 26-0 



10.28 = 26-5 



10.29 = 26-8 



10.30 = 27-0 

 10.36 = 27-4 

 10.45 = 27-4 

 10.55 = 28-3 



At 11.2 the measurements began as follows : 

 Normal doubling periods : 



1. 60-75 121-5 n = 11.2 A.M. 11.37 A.M. = 35 min. at 28 '028 -5 28 '3 C. 



2. 121 -5 243 -0 = 11.37 12.15 P.M. = 38 28 '3 28 -25 28 -75C. 



Or, calculated back : 



252126 n = 11.39 A.M. 12.18 P.M. = 39 minutes at 28 -3 28 '25 28 '75 C. 

 126 63 = 11.3 11.39 A.M. = 36 28-028-5 28 -3 C. 



On August 2 a broth + 1 per cent, gelatine sowing of spores was 

 put in at 8.30, the temperature as follows : 



Temp. 

 Time. C. 



8.30 A.M. = 21-3 



9.30 = 21-4 



10.0 = 21-3 



11.0 = 21-3 



11.15 ,, = 21"5 (opened side window) 



12.0 noon = 21'25 



12.10 P.M. = 21'6 (opened window) 



12.25 = 21-5 



Swollen spore seen. 



12.45 = 21-6 



1.0 = 21-6 Germ. = 3 ft 



1.45 = 21-6 =6/1 



The culture was now put on a slate slab at 18 C. until 4 P.M., when 

 (the box temperature having been raised) it was put into culture at 

 27'5, and raised to 31-2 by 4.40, and measurements made as fol- 

 lows : 



Doubling period : 



135 270 n = 4.40 P.M. 5.17 P.M. = 37 minutes at 31-231-7 C. 



Or, reckoning back : 



283141-5 /t = 4.42 P.M. 5.20 P.M. = 38 minutes at 31-2531 75 C. 



