424 



Profs. Percy Frankland and Marshall Ward. 



It seems to me that a good deal of valuable information might be 

 obtained by pursuing this method. 



On July 30 I repeated this experiment with a rodlet from a two 

 days' culture at 23 25, shaken in its own liquor and then into 

 fresh (broth + 1 per cent, gelatine in both cases). It was started at 

 3.25 at 24 C., and remained till 4 P.M. to accommodate itself to the 

 new conditions, the temperature having risen to 24*6. 



I concluded that no growth occurred either (1) because it took a 

 long time for bacilli beginning to pass over into spore formation 

 to accommodate themselves ; or (2) because the change of tempera- 

 ture was too sudden ; or (3) because something was passed in of the 

 nature of a poison. The shaken tube had remaiaed some hours, 

 and no bacilli in the drop were growing at 5 P.M. 



On July 31 a broth + 1 per cent, gelatine tube, sown 8 A.M. on 

 July 30, was shaken up after having been till 12.30 at 2324 C. 

 i.e., 28^ hours and a sowing made. 



The temperature in Sachs' box was 20*5, and rose as follows 

 during accommodation period : 



Temp. 



I now satisfied myself the rodlets were growing, but they were 

 floating about, and I could not fix one till much later. 



Time. 

 2.25 P.M. 

 2.50 

 3.30 

 4.14 



Temp. 



C. 

 20-25 

 21-0 

 22-1 

 23-25 



