Report on the bacteriology of Water. 425 



One was fixed at 4.18 P.M., and measured as follows : 

 Doubling periods (long beyond 1st normal) : 



1. 54 108 M = 4.18 P.M. 6.4 P.M. = 48 minutes at 23 '523 "75 C. 



2. 108216 = 5.4 5.46 = 42 23 752423 '75 C. 



Or, calculating back : 



279130 '5 n = 5.20 P.M. 6 '0 P.M. = 40 minutes at 23 '923 '6 0. 

 139 -569 75 = 4.38 5.20 = 42 23 -52423 -9 C. 



Another rodlet was chosen at 6.30 P.M., as follows : 

 The doubling period here : 

 103 -5207 M = 6.33 P.M. 7.20 P.M. = 47 minutes at 22 7522 '422 '5 C. 



Or, calculating back : 

 230115 M = 6.40 P.M. 7.30 P.M. = 50 minutes at 22 -6522 -422 -6 O. 



On August 1 a sowing was made of shaken rods from the tube (of 

 rods) made on July 31, arid which had stood 2l hours at 18 20. 



The sowing was made at 9.30, and allowed till 2 P.M. to accommo- 

 date itself, at the following temperatures : 



At 2 P.M. a rod was selected and measured as follows : 



but had to be abandoned. Another was selected at 5.30 as follows 

 [for temperatures between, see spore culture, August 1] in two 

 segments. 



VOL. LV1II. 



2 o 



