Report on the Bacteriology of Water. 



427 



He/diet in Broth. 

 Normal doubling periods : 



1. 33 7567 -5 /* - 6.5 P.M. 6.37 P.M.= 



2. 67 -5135 = 6.37 7.5 = 



32 minutes at 29 -629 "530 '5 C. 

 28 30 -530 C. 



Or, calculated back : 



153-76-5 ju = 6.41 P.M. 7.10 P.M. = 29 minutes at 30 '5 30 C. 



On August 3, rodlets in broth from spores sown at 5.15 A.M., at 

 23 C., and therefore 1\ hours growth were shaken in broth and a 

 culture put into Sachs' box at 27 28 C., at 12.45 P.M. 



During accommodation, the temperatures varied as follows : 



Temp. 



Time. C. 



12.50 P.M. = 2fi-75 



12.52 = 27-25 



12.54 = 27-5 



12.59 .. = 27-75 



1.50 = 27'5 



At 1.54 P.M. the measurements began on a rod 49'5 /t long. 

 49 -599 n = 1.54 P.M. 2.40 P.M. = 46 minutes at 27 '2 28 C. 



Or calculated back : 



141 '7571 n = 2.21 P.M 3.0 P.M. = 39 minutes at 27 '2 28 '5 C. 

 Another rodlet, in two segments, was selected at 3 o'clock. 



Segment A. 



The growth had ceased entirely, and the filament was the same 

 length as at last observation. Whether this was mere arrest, due to 

 falling temperature (as is probable), or death, could not be de- 

 termined. 



2 n 2 



