438 



Profs. Percy Frankland and Marshall Ward. 



by exhaustion or by excreta; the latter is more probable, because 

 much growth went on during the next 24 hours, as examination next 

 day showed. 



On November 24 spores were sown in normal gelatine at 10 A.M. 

 and kept at 22 C. till 3.15. 



Curve 136, p. 434. 

 Doubling periods : 



29 -25 58 -5 p. = 3.20 P.M. 4.12 P.M. = 52 minutes at 21 -621 -7524 -6C. 

 58 -5 117 -0 = 4.12 5.7 =55 21 '6 21 -4 21'6C. 



Calculated back : 



222 -5 111-25 fj. = 5.2 P.M. 6.0 P.M. = 58 minutes at 21 '621 '721 '5 C. 

 111-2555-5 ,, = 4.8 5.2 =54 21 -6 21 -421 -6 C. 



Normal doubling periods : 



40-5 90 /* = 3.45 P.M. 4.46 P.M. = 61 minutes at 21 : 6 21 -4 C. 



90 -0180 = 4.46 ,,5.43 =57 21 -4 21 '7 21-5 C. 



Another culture, November 24, was from spores at 22 C. from 

 1 to 6 P.M. 



Doubling periods : 



1836 n = 6.0 P.M. 7.0 P.M. = 60 minutes at 21 -9 21 -75 C. 

 3672 ,, = 7.0 8.11 =71 21 -75 21 -9 C. 

 72144 = 8.11 9.41 = 90 21 -90 C. 



Normal doubling period : 



2754 (* = 6.30 P.M. 7.42 P.M. = 82 minutes at 21 '7521 '9 C. 



This filament was then left until 8.45, and the following further 

 measurements taken : 



