456 



Profs. Percy Frankland and Marshall Ward. 



The filament had gradually acquired the granulated appearance 

 which comes on when dying. 



It will be noted that this curve is more depressed than the pre- 

 ceding, and that it has nothing like doubled itself in the hour ; still, 

 it is, perhaps, remarkable that growth occurred at all. 



Here, again, we find that during the first hour of exposure to even 

 higher temperatures than on November 5 and 7, it only took twenty- 

 seven minutes to double the length at 36 37'5 C., a range of 1'5 C. 

 (cf. November 5, at a range of 1'25), or at 35'5 to 36'5 (a range of 

 1 C.), whereas during the second hour of exposure it took thirty-one 

 minutes to double, though the range of temperature was only 0'75 to 

 0-9 C., i.e., from 35 35'9 and from 3535-75 C. 



The matter is rendered the more interesting by what occurred 

 during the fifth hour of exposure (10.18 to 11.20 P.M.), for we find 

 that in sixty-two minutes the filament had not accomplished even 

 half the doubling, but had slowly ceased to grow at all. 



It is true, this last case taken alone is open to the objection that 

 the temperature rose so high (35'5 39), but, taken in conjunction 

 with the experience of November 5 and 7, I think it is important. 



Moreover, this experiment of November 9 does not support the 

 suggestion that the gradual slowing is due to the accumulation of 

 CO 2 , or any volatile body inhibitive of the growth and absorbable by 

 KHO. Of course it does not disprove any such hypothesis, but it 

 shows that the slowing is not a mere matter of CO 2 accumulation, 

 and (considering the capacity of my cells and all the conditions) it 

 can hardly be a mere starvation of oxygen supply. 



The results point to intense destructive metabolism, possibly 

 respiratory, as the cause of death, and suggests that the high tem- 

 peratures over-work the machinery of the cells, and, no doubt, the 

 irregular, jerky growth shown on the curves is an expression of this. 

 A.t the same time, it is not impossible that the highly stimulated 

 organism is here so extremely sensitive to minute changes in the 

 environment that these sudden variations are, in part, due to altera- 

 tions not recorded. 



On December 3 spores sown at 2 P.M., normal gelatine at 22 C., 

 were ready at 7.50. 



