470 Messrs. S. G. Shattock and Charles A. Ballance. 



Transference of the Tumour. The malignant tumour was received 

 fresh from the operating theatre, the redundant tissue around was 

 removed with sterilised scissors ; then, with knives previously 

 sterilised at 150 C. for an hour in an iron box, pieces of the 

 growing edge were cut away, and transferred with sterilised forceps 

 to the capsules ; they were laid on the sand just beneath the water 

 level. Two kinds of capsules were used: one, the ordinary Petri, 

 the other considerably deeper, of less diameter, and furnished some- 

 times with a cover, like the Petri, at other times not. 



Storage of the Capsules. The capsules thus prepared and " in- 

 fected " were placed between sterilised double dishes ; the covers of 

 those dishes were raised for a short distance by means of blocks of 

 wood which had been soaked in solution of corrosive sublimate ; the 

 height was such as to allow free entrance of air, but not sufficient 

 to expose the mouth of the lower dish. The double dishes were first 

 sterilised by washing with sublimate solution, absolute alcohol and 

 by heat. 



The double dishes were finally placed, each pair, upon a sheet of 

 glass beneath a capacious shade, both of which had been cleansed 

 with sublimate solution. Most of the small, deep capsules had their 

 covers removed as they were placed between the double dishes. The 

 Petri capsules remained covered throughout. A1J the experiments 

 were conducted in a private laboratory continuously heated. 



Method of Microscopical Examination. A glass rod and slide were 

 sterilised in the flame, and allowed to cool. The shade was removed 

 and the upper dish raised sufficiently to allow of the passage of the 

 rod to the capsule. A little sand was then taken from three or four 

 places along the littoral or from the neighbourhood of the piece of 

 tumour and transferred to the slide ; occasionally a hole was dug 

 with the rod above the water level, and some of the deeper sand re- 

 moved. The sand so removed was gently stroked with the rod on 

 the slide until displaced from one end to the other ; the slide was 

 finally inclined so that enough fluid left the sand to make a micro- 

 scopic preparation. 



The examination was made with 1/12 apochromatic oil immersion, 

 Zeiss, oculars 4 and 8. Occasionally a few drops of beef peptone 

 broth were added to the capsule ; and as the water became low from 

 evaporation more was supplied. 



In all the capsules bacteria developed, a fact which the authors 

 regard as important inasmuch as such would furnish a pabulum for 

 the growth of any protozoa that might develop. 



The authors then give a table exhibiting the results of experiments 

 made in twenty-three capsules ; there were used nine scirrhous 

 carcinomata of the breast, and five sarcomata from different sources ; 

 the sarcomata comprised one from the human biceps, one a mela- 



