472 Attempt to cultivate Parasitic Protozoa from Tumours, $r. 



The authors record, in addition, a certain number of experiments 

 made upon animals by means of sand infected with carcinoma and 

 sarcoma, and containing amoebae which later experiments showed to 

 be adventitious. These experiments, which were all negative in result, 

 included intravenous injection (dogs), the repeated "vaccination" of 

 skin (white rats), intraperitoneal insertion (white rats). 



Having previously found it impossible to raise a growth of 

 carcinoma in any of the lower animals by transplantation of recent 

 human carcinoma, they thought it possible that if the tumour was 

 first incubated outside the body, the hypothetical protozoon might 

 pass into some phase which would enable it to convey the infection. 

 With this object, pieces of carcinoma incubated at the room tem- 

 perature in milk, potassium oxalate plasma, and dilute broth were 

 inserted into the peritoneal cavity of white rats, but with negative 

 result. 



In the case of two rats, the material used consisted of scirrhous 

 carcinoma, which had been buried in a country garden for six weeks ; 

 the animals Avere kept alive for six months, but remained unaffected 

 with the disease. 



Under the head of Treatment are recorded the negative results 

 following the subcutaneous injection of fresh aqueous extract of 

 carcinoma and sarcoma in cases of both these diseases, as well as the 

 similar injection of fresh sheep -seru in. 



