The Antagonism between loxins and Antitoxins. 429 



All weights of venom mentioned below refer to this dried venom. 

 About 2 3 milligrams were weighed out for each experiment and 

 dissolved in 0*9 per cent. NaCl solution, so that 1 c.c. contained 

 O'OOOl gram of dried venom. 



The solution was then heated momentarily to 90 C. in order to 

 destroy one of the poisonous constituents of the venom of this snake, 

 a proteid which coagulates at 85 C.* This was done because 

 Calmette's serum possesses little or no immunising action against 

 this constituent.f 



In all our experiments the same sample of venom was used, and it 

 was treated in the way described above. The injections were always 

 made subcutaneously into the flank. 



From the above series it appears that O000025 gram per kilo, of 

 body weight is about the lowest fatal dose. In the present paper, in 

 speaking of so many fatal doses, this has been taken as the unit. 



V. Experiments to ascertain the value of Calmette's Serum in counter- 

 acting the Poison after it had been deprived by Heat of One Con- 

 stituent. 



The solution of venom was prepared as in Series III. Calmette's 

 serum bore date November, 1896. The two were mixed together in 

 varying proportion, as stated below, and allowed to remain at) 



* C. J. M , < Proc. Roy. Soc., N.S.W./ August, 1896. 

 f C. J. M., ' Intercol. Med. J.,' August, 1897. 



