430 



Drs. C. J. Martin and T. Cherry. 



laboratory temperature (23 C.) for fifteen minutes. They were 

 injected subcutaneously in amounts corresponding to the body weight 

 of the animal. 



2 c.c. of the serum completely counteracts 0'0002 gram of the 

 venom deprived of one of its constituents. 



VI. "Experiments to determine whether mixture of venom and serum 

 (in such proportions as to completely deprive the former of any toxic 

 properties), regained toxic properties after destroying the antitoxin 

 by heating to 68 C. per 10 minute*, subsequent to admixture. 



The venom solution and sernm were mixed in the proportion of 

 1 c.c. of serum to every O'OOOl gram of venom. This proportion 

 was found by the previous series of experiments to be adequate. 

 The two solutions were mixed together, and allowed to remain 30 

 minutes at a temperature of 23 24 C., after which the mixture 

 was heated to 68 C. for 10 minutes to destroy the antitoxin. They 

 were then cooled and injected subcutaneously in varying quantities 

 per kilogram weight of the animal. The injections had no effect 

 upon the animals, although they contained originally eight fatal 

 closes of venom. This must therefore have been neutralised by the 1 

 antitoxin during the time which elapsed before its destruction by 

 heat. 



