256 Prof. V. Harley. Influence of Removal of Large Intestine and 



In the earlier method of experimenting with loops of the large 

 intestine, in some experiments the middle part of the large intestine 

 was isolated, forming a Vella's fistula. 



A given quantity of food was passed into the fistula, and after some 

 hours it was again collected for analysis. By this means, however, I 

 found it impossible to collect the entire quantity put into the fistula 

 unless very large quantities of wash-water were used, which rendered 

 the analysis fallacious. I do not therefore refer in this paper to the 

 results thus obtained, but at the same time wish to draw attention to 

 the fact that in these dogs the Vella's fistula was found in the intervals 

 between experiments to fill up with dflms. For example, in one case 

 the middle (17 cm.) of the large intestine was separated, the upper and 

 lower ends being sewn to the abdominal wound ; the upper and lower 

 part of the divided remnant of the intestine was connected together. 

 The dog at first was fed on milk and gradually on a better diet, as, 

 indeed, all the animals about to be described were treated. The opera- 

 tion was done in this case in March, 1894, and by May, after careful 

 feeding, the dog had gained three pounds in weight, so that the 

 absence of the large intestine had not hindered the animal from 

 putting on flesh. 



The Composition of the Excretion of a Loop of the Large Intestine. 



In May the material that had collected in the loop was analysed, 

 and again on another occasion in December of the next year when the 

 dog was killed. The following are the results of these analyses : 



Analysis of debris collecting in loop of large intestine of dog. 



Fat and 



N. eholesterin. Asli. 

 p. c. p. c. p. c. 



2-473 0-965 

 3-644 2-171 



The analyses above given correspond very closely to those which 

 hare been found to occur in loops of the small intestine. 



Hermann,* Ehrenthal,f Berenstein,| and Fr. Voit, in their 

 examination of isolated loops of the small intestine, showed that 

 they were apt to fill up with contents, which, microscopically and 

 chemically, corresponded with the materials collected from the loop of 

 large intestine in the above dog. 



* L. Hermann, ' Pniiger's Archir,' vol. 46, p. 93, 1890. 



f W. Elirenthal, ibid., vol. 48, p. 74, 1891. 



J M. Berenstein, ibid., vol. 53, p. 52, 1892. 



Fr. Yoit, ' Zeit. f. Biol.,' vol. 29, p. 325, 1892. 



