274 Prof. V. Harley. Influence of 'Removal of Large Intestine and 



Increasing the fat in the diet of this dog did not increase the quan- 

 tity of faeces excreted, and still further did not increase the quantity of 

 nitrogen contained in the faeces as occurred in the normal dogs. Afe 

 the same time it increased the quantity of fat found in the faeces. 



The percentage of nitrogen absorbed under the same circumstances 

 was decreased, but instead of decreasing with the increase of fat as m 

 normal dogs, it practically remains the same, or if anything increases. 

 In fact, the percentage absorption of fat would appear 'to be exactly 

 the same as in the normal dogs. 



The Influence of Complete Removal of the Large Intestine on Metabolism, 



Having now finished the consideration of the dog in which the largo 

 intestine was partially removed, we will next examine the cases of the 

 two dogs in which the whole of the large intestine was entirely- 

 removed together with the caecum. In these instances the small 

 intestine, just above its junction with the caecum, was sewn into the 

 rectum as close to the anus as possible ; in fact, this was in every 

 experiment found by post mortem to be under 6 cm. 



The difficulty in the after-treatment consists in the straining move- 

 merits of the anus being apt to tear the sutures. 



During the first few days after the operation the animal was fed on 

 milk and beef-tea ; later on, the diet was slowly increased, and finally 

 the animal was able to take the normal diet. For some reason or 

 other they were unable to take such large quantities of fat as normal 

 clogs, for when the fat was increased to a certain amount, they either 

 had a severe diarrhoea or refused their food altogether, so that it was not 

 possible to obtain the effect of the marked increase of fat on the com- 

 position of the faeces so clearly as in the normal dogs. 



Dog 4. In dog 4 the large intestine was entirely removed one? 

 month previous to the beginning of the metabolism observations, 

 during which time the diet had been steadily increased as already 

 described, and the animal had begun to feed well on its mixed diet. 

 When its body weight had reached 4 kilos, the dog was then placed on 

 the meat and biscuit diet, and the employment of sterilised meat was 

 begun eight days before the commencement of the analysis. 



The general results are included in Table VI. 



In this case (dog 4) four periods were investigated, two periods in 

 which the amount of fat given was 9 '71 grams, the nitrogen in the- 

 proteid amounting to 6 '80 grams ; in the next two periods the fat was 

 increased to 2 9 '71 grams, while the meat and biscuit diet remained the- 

 same. In this case it was found impossible to increase the fat still 

 more, as the dog then always refused his food. 



(a) During the first period the weight remained constant, and the 

 quantity of water passed fluctuated from 110 to 275 c.c., with a specific 



