from Cancer, and their Pathogenic Effects upon Animals. 433 



Duclaux), the Sac char omycetes are nothing but the developmental 

 stages of fungi which really belong to either the Phyco-, Asco-, or 

 Basidio-mycetes. Moreover, they state that in some species of myce- 

 lium-forming fungi, single parts, especially conidia, can grow in the 

 saccharomyces form on certain nutrient media : so I will not attempt 

 to locate this organism at present. Sanfelice and Eoncali, however, 

 definitely state that the organisms they have isolated are Blasto- 

 mycetes. 



When grown in the medium described, these organisms produce a 

 cloudiness which becomes visible in about forty-eight hours, and 

 increases till about the sixth day, when the growth sinks to the 

 bottom, the medium then becoming clear; no scum or pellicle is 

 formed. 



When grown on this medium solidified with agar, the organisms 

 form small round colonies which remain separate ; after some weeks 

 the colour, which was originally white, becomes yellow ; the colonies 

 do not attain a much greater size than those here shown. 



Gelatin is not liquefied, but the growth on this medium is never 

 luxuriant. On potato, a thick white layer is formed, which in about 

 two weeks will cover the entire surface, changing then to a yellowish- 

 brown colour. 



They will grow aerobically, but not so well, at any rate at first ; and 

 they lose their virulence in a short time if continuously grown in this 

 way. 



Microscopically, they are round bodies, frequently growing in 

 clumps, with a nucleus which stains deeply, and, in most cases, with a 

 thin, strongly refractile capsule, which sometimes shows a double con- 

 tour ; but some young forms can be seen which are apparently with- 

 out a capsule. The size varies from 0'004 mm. to 0'04 mm. 



Their reproduction appears to be by budding, but I have fancied 

 that I have also seen, in a few instances, endogenous budding ; of this, 

 however, I am not certain. 



These bodies correspond morphologically with those found in the 

 original tumour, and also with those described by Kuffer and myself, 

 and by some others of those who have worked at the microscopical 

 appearances of cancer. 



Experimental Results. 



I have selected, from the experimental work which I have done with 

 these organisms, those experiments which seemed to me to be the most 

 important. Up to the present, I have not been able to make any 

 experiments upon such animals as would allow of the easy bringing of 

 these organisms into contact with a likely epithelial surface, with 

 the exception of the cornea (vide Experiment 4 below) ; but, through 



