434 Mr. H. G. Plimmer. Note upon certain Organisms isolated 



the kindness of Dr. Bradford, I have been enabled now, at the Brown 

 Institution, to inoculate a bitch in the mammae, but the time is as yet 

 too short to enable me to make any statement as to the result. 



The cultures used in the following experiments were made in the 

 medium previously described. 



(1) Rabbit. Intravenous injection of 5 c.c. of an eight days old 



culture. 



No obvious result. 



The rabbit was killed thirteen weeks after and found appa- 

 rently normal. 



(2) Rabbit. Intraperitoneal injection of 10 c.c. of a twenty-one days 



old culture. 

 No obvious result. 

 The rabbit was killed eight weeks after and found apparently 



normal. 



(3) Rabbit. Subcutaneous injection of 5 c.c. of a three days old 



culture. 



No obvious result. 



This rabbit was used later for experiment No. 4, and when 

 killed, fourteen weeks after this experiment, was found 

 normal nothing was found at the seat of injection. 



(4) Rabbit. Both corneas were scraped, and the sediment of a ten 



days old culture rubbed over them. 



The rabbit was killed in forty-eight hours. 



There was considerable proliferation of the corneal epithelium, 

 which had forced its way into the subjacent tissue. 



The organisms were found in the epithelial cells, after fixing 

 and staining, with appearances similar to those of the inclu- 

 sions in cancer cells, as described by Buffer and myself. 



(5) Rabbit. Trephined and inoculated beneath the dura mater with 



5 c.c. of a seven days old culture. 



The rabbit died in nine and a half days ; wound healed. 

 The brain and cord contained the organisms in large numbers. 



Pure cultures were obtained from brain, liver, and kidney. 



Nothing obtained from blood, spleen, or peritoneal fluid. 



(6) Rabbit. Trephined and inoculated beneath the dura mater with 



5 c.c. of a three days old culture, made from brain of No. 5. 



The rabbit died in eight days ; wound healed. 



Organisms found in heart, blood, brain, and cord. Pure cul- 

 tures made from brain, cord, kidney, and liver. 



(7) Guinea-pig. Subcutaneous injection of 5 c.c. of a ten days old 



culture. 



No obvious result. 



The guinea-pig was killed in fifteen days, and was found appa- 

 rently normal ; nothing was found at seat of injection. 



