from Cancer, and their Pathogenic Effects upon Animals. 



(8) Guinea-pig. Intraperitoneal injection of 10 c.c. of a three weeks 



old culture. 

 Died in twenty days. 

 Liver, lungs, and peritoneum studded with new growths of a 



white colour ; lymphatic glands in abdomen enlarged. Pure 



cultures made from liver, lungs, and abdominal glands ; 



nothing obtained from blood. 

 Sections of the above mentioned parts showed new growths of 



an endothelial nature, with the organisms within the cells, 



and free in the tissues. 



(9) Guinea-pig. Intraperitoneal injection of 10 c.c. of an eight days 



old culture, made from abdominal glands of No. 8. 

 The guinea-pig died in seventeen and a half days, and showed 



the same appearances as No. 8. 

 Cultures were made as before, and also from the blood. In this 



case the omentum was also studded with new growths. 



I have given here some of the failures and successes which have been 

 constant ; and I should like to add that Professor Wright, of Netley, 

 has repeated some of the experiments I have made, and that his results 

 coincide with mine. 



The important point, of course, of all this is the experimental pro- 

 duction of malignant tumours in animals by an organism isolated from 

 a malignant tumour in man. That these experimental tumours are, so 

 far, of endothelial origin is due to the fact that until I was enabled 

 to inoculate a dog, I found it very difficult to get the organism in con- 

 tact with likely epithelium ; all the above methods of inoculation, save 

 one, could only bring them into contact with endothelial surfaces. 

 No. 4 (the corneal experiment) is the only one in which an epithelial 

 surface was tried; and in this case the great proliferation of the 

 epithelium, the appearances of the organisms in the cells, and the 

 irritation produced, are very striking. But the fact of being able to 

 excite a malignant growth with an organism isolated from cancer is, I 

 think, a point of some importance in the aetiology of cancer. 



I am at present experimenting with the view of observing the effects 

 produced by these organisms when brought into contact with epithelia. 



The deductions which I think may fairly be made from these obser- 

 vations and experiments are as follows : 



(1) That there are certain cancers, which occur very rarely, in which 

 there are, in enormous numbers, intracellular bodies of the kind 

 described by Ruffer, myself, and others, as parasitic Protozoa. (From 

 the rarity of these cases and their comparatively acute course, one is 

 tempted to think that they are not due to the same cause as ordinary 

 cancers j but there is really no more difference between them and 

 ordinary cancers than between acute and chronic tubercle.) 



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