446 Dr. C. W. Daniels. On Transmission of 



examined, three were reserved for sections, one was too much 

 decomposed for satisfactory examination ; ten were not 

 accounted for, having been lost in the nets. 



Of the forty-nine examined two were killed on the first day, that 

 is, under twenty-four hours, and possibly under twelve hours after they 

 had fed ; no coccidia were found in these. Two more were examined 

 the following morning, that is under thirty-six and possibly under 

 twenty-four hours after they had fed ; no coccidia were found in these. 



In two examined about 4 p.m. of the same day, that is, under forty- 

 six and possibly not more than thirty-four hours after they had fed on 

 the infected birds, minute pigmented coccidia were found. 



The remainder were examined on the following days. The largest 

 numbers (eighteen) w^ere examined on the fourth and (twelve) on the 

 seventh days, as on these two days the mortality amounted to this. 



In all these mosquitoes, with one exception, coccidia were found 

 usually in numbers ; in one there was only one coccidium. 



The exception occurred on the ninth day ; but as by that time the 

 insects had been re-fed several times, the mosquito in question may 

 have been an outside one which had effected an entrance. 



Of forty-five mosquitoes fed on the infected birds and examined, 

 more than thirty-four hours after, forty-four contained coccidia. 



This I may say is a more successful result than in the other series I 

 have seen. 



The other two series of mosquitoes were used by all of us for the 

 preparation of specimens, and no record was kept of the number of 

 non-infected insects. Judging from my own examination, only about 

 three-quarters of them developed coccidia. Their treatment had been 

 somewhat different, as for several days half of them were not in- 

 cubated. 



Of the controls fed on birds free from Proteosoma, thirty-eight in 

 number, and treated in the same manner, twenty-nine were examined 

 and nine are unaccounted for lost in the nets. None of the 

 twenty-nine were examined on the first day, but one was on the after- 

 noon of the second day. The largest number, seven and five, were 

 examined on what would correspond to the fourth and seventh days, 

 four were examined on the fifth and four on the sixth days.* In none 

 of these twenty-nine were coccidia found. 



Of the eighteen fed on the blue jay with halteridia, twelve were 

 examined from two to six days after feeding; none contained 

 coccidia. 



* It will be observed that these control mosquitoes were not, as the other series, 

 collected on one, but on three nights. A very slight difference in breeze and light 

 seems to affect the numbers that feed ; any extra restlessness on the part of the birds 

 has the same result. 



