'294 Mr. A. l-Miii-toM. Xouth African Hur* -xicL-nes : 



phenomena are, therefore, for the most part to be ascribed to the 

 marvellous exudation of blood plasma, which, while seen more or less 

 throughout the serous and subcutaneous tissues, is best marked within 

 the thoracic cavity. 



In my annual reports as Director of the Colonial Bacteriological 

 Institute I have referred to the morbid anatomy in greater detail. 



Inoculation Experiment*. 



For the purpose of conveying to healthy animals the infection from 

 those already sick three materials have been made use of, viz. 



1. The yellow fluid from the trachea of infected animals. 



2. The yellow fluid from the pericardium of infected animals. 



3. The blood of infected animals. 



The use of the first two fluids has not always been successful in 

 setting up the disease, but fresh virulent blood has invariably proved 

 successful. 



of Use of the Material* mentioned. 



(<t.) By subcutaneous injection. 



(b.) By insertion of a seton, impregnated with the fluid, under the 

 skin. 



(c.) By drenching, e.r/., giving a dose by the mouth. 



Sites Selected. 



(a.) Directly into the lung tissue by hypodermic needle operating 

 through the skin over an intercostal space. 



(b.) Into the subcutaneous tissue of the neck. 



(c.) Into the subcutaneous tissue of the flank. 



(</.) Intravenously. 



Any one of the channels selected is equally suitable, but the incuba- 

 tion period is somewhat shorter when the intravenous method is used. 



Period of Incubation. 



When the malady is induced by the inoculation of 2 or 3 c.c. of the 

 blood of an animal which has died from spontaneous disease, a mean 

 period of eight to nine days supervenes, after which the temperature 

 begins to rise. The elevation is gradual, with remissions during the 

 night, but attains to 106 F., as a rule, before death, which usually 

 occurs after four. or five days of fever. 



