its Patlwloyy and Method* of Protective //w/'A///n,/. 297 



(r.) Goat*. Angora goats are also to a limited extent susceptible to 

 horse-sickness infection. Of seventeen which were inoculated, a 

 febrile reaction occurred in ten; none died. From one of the ten 

 blood was taken, which was used to inoculate an ox. The latter 

 animal developed fever, and died with exactly similar symptoms 

 during life, and showed the same post-mortem conditions as the ox 

 already referred to, whose blood, when inoculated into a horse, pro- 

 duced characteristic horse-sickness. 



(d.) Sheep. Sheep are also susceptible. Of ten which were inocu- 

 lated, six showed a well-marked febrile reaction, but none died. 



I have not succeeded in transferring the disease to rabbits, guinea- 

 pigs, rats, or mice. 



The Transmission of the Disease for Protective Inoculation by Means of the 

 Inoculation of Fresh Blood. 



The inoculation of horses with the blood of donkeys which were 

 suffering from fever produced by inoculation has been attended with 

 most varying results. 



In some cases death has been produced, in some an irregular febrile 

 period, while in others no apparent result has followed. The period of 

 onset of the fever has likewise been most variable. In some cases a 

 reaction has been set up corresponding to the normal period of incuba- 

 tion which obtains in horse-sickness, while in other cases reaction has 

 been delayed for more than 25 days. 



Influence of the Reaction produced. 



Where fever has set in on or about the eighth day, been moderately 

 severe in degree and duration, and subsequently subsided, a very 

 definite degree of protection has been produced, although seldom high 

 enough to set up such a resistance as to oppose death when the animal 

 was subsequently inoculated with virulent blood. 



A striking demonstration of variable susceptibility among horses 

 was furnished during these experiments. Of three horses and one 

 mule which were each inoculated subcutaneously with 5 c.c. of fresh 

 blood 



The mule had no reaction. 



Two horses had scarcely any reaction. 



One horse had a good reaction. 



In the case of the last horse, when subsequently inoculated with 

 virulent blood it suffered severely and just managed to recover. The 

 others had not been protected to any appreciable degree. Obviously, 

 therefore, the susceptibility of the last animal had been such as to 

 admit of infection from the donkey's blood producing reaction and the 



