its Pathology and Methods of Protective Inoculation. 303 



1st Inoculation 1 c.c. virus and 100 c.c. serum (10 animals). 

 1 c.c. ,, 90 c.c. (4 animals). 



0'5 c.c. ,, 50 c.c. (2 animals). 



r A slight variation 



2nd Inoculation 0'5 c.c. virus in 30 c.c. ,, I of the quantities 

 3rd Inoculation 0'5 c.c. 15 c.c. | was made in 



*>- several cases. 

 4th Inoculation 5 c.c. pure preserved virulent blood. 



The following shows the results obtained, and where the remark 

 "salted" is made, it is to be understood that the animal has, at later 

 dates, withstood enormous doses of the most virulent blood. 



Animal. Eeaction. Result. 



1st. No reaction at all Salted. 



2nd. Reaction to 1st only 



3rd. No reaction ,, 



4th. Slight reaction after all four 



5th. Eeaction to 4th Died. 



6th. Slight reaction to 1st 



7th. No reaction Salted. 



8th. Reaction to 4th Died. 



9th. Slight reaction to 3rd; after 5th... 



10th. Slight reaction to 4th Salted. 



llth. Slight reaction to 4th ,, 



12th. Reaction to 5th Died. 



13th. No reaction Salted. 



14th. Reaction to 5th 



15th. Reaction to 4th 



16th. Reaction to 4th ,, 



In the next experiment seven animals were used, which were inocu- 

 lated as follows : 



Inoculations. 



1st. 1 c.c. virus and 100 c.c. serum. 



2nd. 0-5 c.c. 25 c.c. 



3rd. 0-5 c.c. 10 c.c. 



4th. 0'5 c.c. 1'5 c.c. 2 had 0'5 c.c. pure virus. 



The results were as follows : 



Animal. Reaction. Result. 



1st. Slight reaction to 5th Salted. 



2nd. ,, 3rd and 5th. 



3rd. No 4th After a large dose of pure 



virus, died. 



