."i<>4 Mr. A. Kdiiiirti'ii. - s // 



Animal. 'ion. Result. 



4th. Slight reaction to 2nd Salted. 



~)th. No 4th After a large dose of pure 



virus, died. 



T.th. Slight .. -2nd Salted. 



7th. .. 3rd 



Where " 5th " is mentioned, it refers to a dose of pure virulent 

 blood. 



Total animals inoculated 23 



,, died 9 



salted 14 



The tests thus applied have been of the most severe character, and 

 despite the fact that these are only of the value of preliminary experi- 

 ments, the results are extremely satisfactory. 



Obviously animals have been sacrificed which, under altered methods, 

 might have been saved, for the outcome of these inoculations goes to 

 show that, unless some reaction has been produced during the earlier 

 reactions, there is no certainty that an animal is protected. Never- 

 theless it is equally proved that some have become highly protected 

 without having shown any reaction at all. 



The indication, therefore, has been to increase the dose of the virus 

 used in the primary inoculations, even at some risk to the more 

 susceptible animals. 



In a subsequent series of animals this has been carried out in the 

 following manner : 



1st. Inoculation 2 c.c. virus and 50 c.c. serum. 

 2nd. 2 c.c. 20 c.c. 



Twelve animals have been simultaneously inoculated in this manner. 

 The reactions produced have been as follows : 



Animal. 1st Inoculation. 2nd Inoculation. 



1 None. None. 



2 Slight. Slight. 







4 Severe. 



5 Slight. None. 



6 ,, 



7 Severe. Slight. 



8 Slight, 



9 None. 

 10- Slight. 



11 None. 



12 Severe. 



