STUDY OF GRAPE POLLEN. 



245 



Barry and Wyoming had not changed in any way from their condition 

 when they were first placed in the solution. About 12 per cent, of the 

 Mo. Reisling germinated 20 per cent, of Rogers' No. 32 and 10 per cent, of 

 the Lucile. Pollen of these same varieties was placed at this later date in 

 10 per cent, dextrose solution with the following results : Grein Golden out 

 of an estimated 200 grains one made a very weak growth. Barry and Wyo- 

 ming not changed in any way. Mo. Reisling, about 15 per cent, germinated. 

 Rogers' No. 32, about 50 per cent, and especially strong. Lucile was acci- 

 dentally destroyed. The same varieties were tried in 10 per cent, sugar 

 solution with the following details : Grein Golden, out of an estimated 150 

 grains one germinated, growth short and weak. Mo. Reisling, 20 per cent, 

 grew. Barry, none. Rogers' No. 32, about 75 per cent, good and strong. 

 Lucile is a mass of growth at least 80 per cent. In 20 per cent, sugar, Grein 

 Golden shows 2 in approximately 250. Mo. Reisling, 25 per cent. Barry, 

 none. Rogers' No. 32, 90 per cent Wyoming, about 5 per cent., growth 

 weak. Lucile, 95 per cent. 



On July 2 cultures were made in 20 per cent, sugar solution with pollen 

 of Black Eagle, Triumph, Massasoit, Brilliant, Roscoe, Lindmar, Hexamer 

 and Bailey. Notes taken the following day show : Black Eagle, none budded 

 or grown. Triumph, practically all budded but only about 10 per cent, grown 

 to any length. Massasoit, little budding and no growth. Brilliant, all budded, 

 60 per cent, grown. Roscoe, none budded and none grown. Lindmar, about 

 60 per cent, budded and 10 per cent, grown. Hexamer, no buds and no 

 growth. Bailey, about 60 per cent, budded and 5 per cent grown. The 

 foregoing results are tabulated below : 



None 



4% 

 None 



10% 

 1% weak 



25% 

 None 

 90% 



5% 



95%, very strong 

 None 



10% 

 None 



60% 

 None 



10% 

 None 



5% 



Besides these there were some other differences between the self-strile 1 

 and the self-fertile pollen which seemed to be constant. The self-fertile 

 grains seem to be surrounded by a mucilaginous substance which makes 



*! retain the use of these terms self-sterile and self- fertile all through this bul- 

 letin, although the results show they (Jo not express the whole truth. 



