136 Dr. C. Bolton. On the [July 26, 



Action when injected into the guinea-pig. 

 Symptoms following injection. 

 Post-mortem lesions in guinea-pig. 

 Nature of the gastrolytic serum. 

 Effects of heat. 

 Specificity of the serum. 

 Kelation to hsemolysin. 

 (c) Changes in the rabbit's peritoneum. 



(3.) Effects of Injection of the Stomach Cells of the Rabbit into the 

 Rabbit. 



Changes in the rabbit's blood. 



Action on injection into the rabbit. 

 Action on injection into the guinea-pig. 

 Effects of heat. 

 Effects of previous treatment with guinea-pig's 



stomach cells. 

 Effects of previous treatment with rabbit's 



stomach cells. 



(4.) Effects of Injection of the Stomach Cells of the Guinea-pig into 

 the Guinea-pig. 



Action on injection of the serum into the rabbit. 

 (5.) Action of Normal Rabbit's Serum on Injection into the Guinea- 

 pig- 



(6.) Action of Hsemolysin on Injection into the Guinea-pig. 

 (7.) Lesions in the Guinea-pig's Stomach due to Causes other than 



Haemolysin or Gastrolysin. 

 (8.) Conclusions. 



(1.) METHODS. 



Preparation of Mucous Membrane for Injection, The instruments used 

 for this purpose are a knife, a porcelain plate, a glass mortar, and a 

 10 c.c. syringe, all of which can be sterilized with the greatest ease. 



A guinea-pig which has been previously starved for about 24 hours, 

 so that its stomach is empty, is killed with chloroform and the stomach 

 at once cut out. It is opened and thoroughly washed in a stream of 

 sterilized 0'86-per-cent. salt solution, all the adherent mucus being com- 

 pletely removed. The mucous membrane is then scraped off and 

 ground up into an emulsion with salt solution, which is then injected 

 into the peritoneal cavity of a rabbit. I have also used subcutaneous 

 injections and have obtained the same results, but as suppuration is so 

 liable to occur I have discarded them. 



In two cases a fresh filtered extract of the mucous membrane was. 

 used for intraperitoneal injection. In one of these cases a 0'1-per-cent. 

 hydrochloric acid solution and in the other a 0'4-per-cent. sodium 

 bicarbonate solution was used for making the extract. The mucous 



