138 Dr. C. Bolton. On the [July 26, 



the animal prostrate. This is simply a vascular phenomenon due to 

 the shock of injection, and the condition is soon recovered from, the 

 animal subsequently taking its food well, and seeming to be quite 

 healthy. Sometimes there is a fall in body weight, but this is by no 

 means constant. 



I have not found that any toxic symptoms follow the injection, and 

 if the animal dies it is the result of bacterial infection. 



(b) Changes in the liabbit's Blood. 



After four or five injections at intervals of 10 days, the blood serum 

 of the rabbit is found to possess toxic properties. 



Action in Vitro. Contrary to the results of several observers, who 

 have found that many tissue cells become dissolved in their corre- 

 sponding cytotoxic sera, I have never been able to detect any difference 

 whatever between the glands which had been exposed to the gastrolytic 

 serum and those which had been similarly treated with normal rabbit's 

 serum, although a marked effect was obtained on injecting the former 

 serum into guinea-pigs. 



In those cases in which the glands had been exposed for long periods 

 of time to the two sera, each showed an equal degree of maceration in 

 proportion to the time of exposure. 



Action on Injection into Guinea-pigs. 



Symptoms following Injection. In about half-an-hour after intra- 

 peritoneal injection of the serum the symptoms are well marked. The 

 animal sits huddled up, with hair erect, and will not move. The 

 temperature becomes subnormal. 



If the dose of serum is small (15 c.c.), the animal will probably 

 have quite recovered by the following day, but with large doses (10 c.c.) 

 t becomes rapidly worse, and finally general twitchings commence, and 

 the animal lies prostrate, death occurring generally within 24 hours 

 In one case a guinea-pig weighing 280 grammes was killed by 5 c c , 

 m another an animal weighing 140 grammes by 1 c.c., but, as a 

 ile, the killing power of the smaller doses is uncertain. It may be 

 ed that in most cases a dose of 10 c.c. is fatal for a guinea-pi^ 

 weighing from 200-300 grammes within 24 hours 



All guinea-pigs which are killed by the serum invariably show lesions 



stomachs, and a large proportion of those which receive small 



1 recover show similar lesions. Speaking generally, the larger 



The le ad 1 mimstered ' the grater- is the effect upon the stomach. 



Lhe lesions always occur during the first 24 hours after injection. 



^c Appearance of Lesions in Stomach.~The lesions consist 



.~ esons conss 



AlUh foTlow "T 0818 ^ *? mUCOUS membrane > and h-norrhage. 

 3 following lesions are described from cases which survived and 



