141' 



Dr. C. Bolton. On the [July 26, 



added to it and the mixture kept at laboratory temperature for 1 hour. 

 The corpuscles were then centrifugalised off and normal rabbit's 

 serum added to the supernatant fluid. On injecting this mixture into 

 a guinea-pig stomach lesions resulted. 



It was proved that the corpuscles had extracted the haemolytic 

 factor, because on adding to the heated serum some guinea-pig's 

 corpuscles and normal guinea-pig's serum, complete laking resulted, 

 whereas on adding the corpuscles and normal serum to the gastrolytic 

 serum, which had been treated with red corpuscles previously, no 

 laking or only a slight discoloration of the serum immediately above 

 the deposited corpuscles was visible after 24 hours. 



Rabbits Stomach Cells. These cells also failed to extract the 

 gastrolytic amboceptor. As will be seen later, this experiment stands 

 on a different footing from the last three, and the fact of the cells 

 failing to extract the amboceptor does not prove that they do not 

 possess an affinity for such amboceptor. 



delation to Hcemolysis. The specificity of the gastrotoxin was also 

 tested by immunising a rabbit against washed guinea-pig's stomach. 

 The washing was carried out by passing a cannula into the thoracic 

 aorta, cutting the inferior vena cava above the diaphragm, and then 

 allowing a stream of sterilised salt solution to flow through the cannula 

 until the blood was completely washed out of all the abdominal organs. 

 This experiment is quite easily accomplished. 



The serum of this rabbit showed a great increase in its hsemolytic 

 power after it had received three injections of stomach cells, and the 

 serum on injection into a guinea-pig caused necrosis of the stomach. 

 This experiment affords confirmation of the fact, which was first 

 demonstrated in the case of spermatozoa by Moxter and in the case 

 of ciliated epithelium by Yon Dungern, that different kinds of cells 

 may contain similar receptors. 



In the present instance the gastric cells not only contain specific 

 receptors peculiar to themselves, but they also contain receptors 

 similar to those of red blood corpuscles. Whether other cells of the 

 body may contain receptors similar to those which I have stated as 

 being peculiar to the gastric cells the above experiments do not 

 conclusively prove. So far as they go the experiments prove that, 

 at any rate if present in other cells, the gastric receptors are not so 

 abundant in them as in the gastric cells themselves ; other cells may 

 contain a few of those receptors, but not sufficient to extract all the 

 immune body contained in the serum. 



At any rate, in the present state of our knowledge, these experi- 

 ments tend to show that the gastrotoxin not only contains a specific 



iboceptor, which has an affinity for the receptors peculiar to the 



trie cells, but also an amboceptor having an affinity for the 

 receptors which the gastric cell possesses in common with red blood 



