144 Dr. C. Bolton. 0w the [July 26, 



(3 ) EFFECTS OF INJECTION OF THE STOMACH CELLS OF THE 

 KABBIT INTO THE KABBIT. 



The same initial symptoms, and the same changes in the rabbit's 

 peritoneum, are observed as in the case of injection of guinea-pig's 



stomach cells. 



Changes in the Xabbit's Blood. I have been unable to produce any 

 stomach lesion, or demonstrate any toxic action, on injecting the 

 serum into a rabbit. 



A<-tinn on Injection into the Guinea-pig. That the blood has acquired 

 toxic properties, however, I have shown by injecting the serum into 

 guinea-pigs. Identical lesions are obtained by such injections with 

 those described above. 



Effects of Heat. My experiments so far tend to show that, on 

 heating the serum to 5060 C. for 1 hour, the action is destroyed if 

 the serum has only a low toxicity, but that some action may remain 

 in the case of the more toxic sera. According to these results, the 

 <ruinea-pig serum may to some extent complement this amboceptor. 



Effects of Previous Treatment with Guinea-pig's Stomach Cells. The 

 stomach cells extract the amboceptor, and render the serum inactive, 

 as described in dealing with the serum obtained by injecting the 

 rabbit with guinea-pig's cells. 



Effects of Previous Treatment with Rabbit's Stomach Cells. In this case 

 the rabbit's stomach cells entirely fail to anchor the amboceptor, and, 

 on injecting the serum thus treated into a guinea-pig, necrosis of the 

 stomach results. The same result was seen in the serum of rabbits 

 immunised with guinea-pig's cells. 



From these experiments I think it may be inferred that the rabbit 

 can produce a gastrolytic serum when rabbit's stomach cells are 

 injected into its body. And also, that the immune body thus pro- 

 duced has two- cytophilic affinities : (1) for the receptors of the 

 rabbit's stomach cells, since it is formed in response to their injection ; 

 (2) for the guinea-pig's stomach cells, since it causes necrosis of them. 



Another alternative would be to assume that two separate immune 

 bodies are formed. Whichever of these two views be accepted, the 

 conclusion follows Jthat the failure of the rabbit's stomach cells to 

 anchor the immune body for which they have an affinity points to 

 the hypothesis that this junction is prevented by the interposition of 

 an anti-immune body, this anti-immune body having presumably been 

 formed by the rabbit to protect its own stomach and prevent 

 autolysis. 



If we accept the view that two separate immune bodies are formed, 

 we must also conclude that the rabbit's stomach cell has receptors 

 similar to the: guinea-pig's, as well as those peculiar to itself ; this 

 view presents the difficulty that we cannot explain why the guinea- 



