162 Dr. Wright and Capt. Douglas. Action on [July 2Q 



Experiment 2. 



A. 



S. R. D.'s unheated serum 2 vole. 



S. R. D.'s washed corpuscles 2 



Suspension of living tubercle bacilli 1 vol. 



Phagocytic index (average of 16 P.W.B.C.), 17'3. 



B. 



S. R. D.'s serum heated to 60 C. for 20 mine 2 vols. 



S. R. D.'s washed corpuscles '< 



Suspension of living tubercle bacilli 1 vol. 



Phagocytic index (average of 37 P.W.B.C.), 3'0. 



Experiment 3. 



A. 



C. J.'s unheated serum 2 vols. 



S. R. D.'s washed corpuscles 2 



Suspension of living tubercle bacilli 1 vol. 



Phagocytic index (average of 19 P.W.B.C.), 14. 



B. 



C. J.'s serum heated to 60 C. for 20 mins 2 vole. 



S. R. D.'s washed corpuscles 2 



Suspension of living tubercle bacilli 1 vol. 



Phagocytic index (average of 37 P.W.B.C.), 3'0. 



With cultures which have been exposed to a temperature of 100* C. 

 precisely similar results are obtained, while an advantage is gained in 

 the respect that the count is no longer rendered difficult by the massing 

 together of the bacilli. 



All the experiments hereafter subjoined have been carried out with 

 such a culture, i.e., a culture heated to 100, broken up in l-in-1000 

 NaCl solution, and centrifugalised until all unresolved clumps had been 

 carried down. 



Our next series of experiments was undertaken with a view to 

 determining whether the increased phagocytic effect obtained with the 

 unheated serum is due to an action exerted by the serum directly upon 

 the tubercle bacilli. 



Experiment 1. 



A. 



S. R. D.'s unheated serum 2 vols. 



A. E. W.'s washed corpuscles 2 ,, 



Suspension of heated tubercle bacilli 1 vol. 



Phagocytic index (average of 20 P.W.B.C.), 6*9. 



