172 Dr. Wright and Capfc. Douglas. Action on [July 2( 



Pr'tii'-ijih upon which the Patients were Selected and General Procedure 

 Followed in Connexion with the Inoculations. 



We have in our selection of cases been guided by the desire to deal 

 at first only with the most aggravated and seemingly intractable cases 

 of localised tubercular infection, and only with cases which would furnish 

 unambiguous objective evidence of any progress or regress of the 

 infection. 



The general procedure followed was to begin in each case asfter 

 the measurement of the content of the patient's blood in tuberculo- 

 tropic substances, with very small doses (generally 1 /500th milli- 

 gramme) of the vaccinating material, and to reinoculate at intervals of 

 10 days, retesting the blood on each occasion, and in the case of each 

 patient expressing the results in the form of a curve. 



In our earlier experiments, before we had elaborated the procedure 

 for measuring the opsonic power of the blood, we were necessarily 

 restricted to a measurement of the agglutinating power. 



Data Furnished by the Measurement of the Agglutinating Power in the 

 Case of Patients Undergoing Anti-Tubercle Inoculation. 



The method of investigation here in question and we would note 

 that it had before us been employed by Koch in connection with 

 inoculations of his T.E. tuberculin furnishes, it seems to us, indications 

 which have a certain value. 



An increase in the agglutinating power of the blood is generally, as 

 in the cases tabulated below, obtained in the course of a successful 

 immunisation. 



It is, however, to be noted that the rise in the agglutination curve 

 may occur long subsequent to the achievement of very marked clinical 

 improvement, and further that such clinical improvement may be 

 obtained quite apart from any sensible increase in the agglutinating 

 power of the blood. 



